Techniques for Diagnosis of Specimen

Techniques for Diagnosis of Specimen Question 1 Describe how your laboratory would deal with this specimen? When the liver core specimen in formalin arrive at the Histology laboratory lab, the first thing that the staff would do is the specimen accessioning, which the specimen is being accessioning y labelled it with number in order to identify each of the specimen for each of the patient (Edward C., 2013). Since the specimen arrived already been fixated with formalin in order to prevent it from decaying and also at the same time try to preserve the cells and tissues to be in a â€Å"life-like† state (Rolls Geoffey, 2011). After fixation, the next step would be grossing or known as â€Å"cut-up†, in which in this case the liver specimen need to be dissect into small pieces in order to be fit into the cassettes. After the specimens being put in the cassettes, it is then being load onto the tissue processor for processing through the wax. Tissue processors are used for preparation of paraffin section (Rolls Geoffey, 2011). It will allow the specimen to be in a molten paraffin wax state after a sequence of different solvents infiltrated in the specimen (Rolls Geoffey, 2011). Embedding is the next step where after processing, the specimen is being placed in an embedding center, placed in wax filled molds after it being removed from the cassettes (Rolls Geoffey, 2011). This is the stage where the liver specimen is being carefully oriented because in order to see the abnormal area to be visible under the microscope and thus determine the plane thoroughly (Rolls Geoffey, 2011). Using the appropriate block, the specimen is filled with wax and let it solidify for a few minutes (Rolls Geoffey, 2011). A stable base has been provided for clamping in the microtome when the cassette is now filled with wax and forming as part of the block (Rolls Geoffey, 2011). Now, section cutting process can be preceded. Section cutting is been done by using the instrument called microtome by using an extremely fine steel blade (Hubscher, S., 2008). To get only a single layer of cells that is makes up the section, the paraffin sections are usually being cut at a thickness of 3 – 5  µm (Hubscher, S., 2008). Since sectioning making it in a form of ribbons since they will stick together edge to edge making it easier to picked up onto the microscopic slides on the floated sections on the surface of warm water in a flotation bath in order to flatten them (Hubscher, S., 2008). The specimen are ready for staining after a thoroughly drying (Hubscher, S., 2008). Question 2 Which specific test could be performed within your histology laboratory to assist in the diagnosis? Why did you choose these? Based on the received liver core specimen, it shows that the total iron body content is quite high which about 20gm. Thus, the most specific test to assist in the diagnosis of the iron concentration is Perl’s Prussian blue test (PPB). Perl’s Prussian blue stain is the major histochemical stain that is used to detect as well as identified the ferric iron (Fe3+) in particular tissues [4]. By the principle of hydrolysis of mineral acid, this ferric ion then is being released from the deposition of tissues, which are protein bound. Based on chemistry’s concept itself, iron in ferric state wills formed ferric ferrocyanide since it is reacted with hydrochloric acid. Thus, this makes the insoluble blue compound visible [5]. There is no colored product by ferrous ions themselves, thus their reaction cannot be seen. Within this technique, usually red in color will represents a nuclei in that particular normal cell, yellow in color for erythrocytes, deep blue stained for ferric salts and lastly, stained as blue or black for the asbestos bodies. The stain is composed of aqueous hydrochloric acid, aqueous potassium ferrocyanide and a neutral red stain[6]. Measuring 10ml of hydrochloric acid, which is about 2% in concentration, starts the staining method. Then, 2% of potassium ferrocyanide was added and ensure that the mixture mixed thoroughly by shaking hem well. Next, placed the slides on the rack for staining step and carefully filtered the solution onto the slides and leave it with that solution for about 15 minutes. Later, carefully removed the excess solution from the slides by rinsed it using the distilled water. The step continued by filtered 1% of neutral red onto the slides and leaves it for about 5 minutes. The purposed of this step is to let the slides for having the capable time so that it will be able to attach to the dye completely [7]. About 5 minutes later, thoroughly rinsed the slides by using distilled water to remove any excess dye, which has not been attached to the cells components. The washing step should not be decreased below 5 minutes as thorough washing is required to prevent a heavy dye precipitate resulting from the neutral red counterstain [8]. The slides are then are blotted by using filter papers. Quickly, the slides were rinsed in 70% of industrial methylated spirit and at the same time agitating the slide by making a slosh up and down. Then, placed the slides in absolute industrial methylated spirit (100%) and further agitated for another one to two minutes. Finally, always placed the slides mount in a DPX-type mountant since other mounting media results in fading of the stain. Again, the slides were agitated for about 2 minutes to ensure that there was no gas trapped in the slide [9]. Then, covered the slides with cover slips by placing a mountant upon the cover-slip which is to cover the section on the slide and the slide removed from DPX and then was gently touched on the side of the slide with the section to the cover slip. Question 3 What are the expected results from the staining methods you have chosen? The pattern of iron deposition can be obtain with the help of iron staining.4 The iron stain shows the features of the possible cause of excess iron in the body. 4 The degree of the iron deposition and many grading methods exist to grade the extent of deposition in the liver by using iron stain.4 The main organs that store excess of iron is the liver. Iron stored in cell in soluble compound is called ferritin while insoluble form is called hemosiderin. Only hemosiderin that can be seen using HE stain while the ferritin cannot be seen. The hemosiderin appears as coarse golden brown refractile granules. On the Perls Prussian Blue stain, the ferritin appear as a fain bluish blush and the hemosiderin appears a deep blue in color.4 Figure 1 The liver core specimen1,2 This is the result obtain from the patient’s liver core specimen. It can be seen in the Figure 1 of large blue granules mark. This is how the presence of iron in the cell. The nuclei pigment appears red and the iron pigment appears deep blue in color with the Prussian blue stain. This means there is excess of iron stored in the liver of this patient.3 For a normal human being, excess of iron is stored in the year. Increase of the ferric iron stores can be identified as Hemochromatosis. The excess iron in body can lead to increase iron store in the liver. The iron is stored in the intracellular compartment of the liver.2 Question 4 What additional non-histological tests would you recommended to the following clinican? For the additional non-histological tests, serum ferritin blood test is recommended to be used by clinician in order to detect the content of iron in patient’s body (Adams P, 2008). It is an enzyme-linked assay that can be performed on blood sample from nonfasting patient (Adams P, 2008). 70% of the total iron store in patient’s body can be found in her haemoglobin while the 20% of the total iron stored as ferritin (Hicks R, 2013). The increase or decrease of ferritin level will indicate the changing of iron level in her body (Hicks R, 2013). The normal serum ferritin level is not more that 200ng/ML in women and normal iron level in human body is about 5 gram (Hicks R, 2013). Firstly, by referring to the Figure 2, the serum ferritin blood test will be started by drawing the blood from a vein of the patient (KidsHealth , 2014). The surface of patient’s skin will be cleaned with antiseptic and the tourniquet (elastic band) is placed around the upper arm to cause blood swell in the targeted vein (KidsHealth , 2014). Then, a needle will be inserted into the targeted vein causing the blood to withdraw from the vein and collected in a syringe (KidsHealth , 2014). When the procedure is completed, the elastic band is removed from the patient’s body part (KidsHealth , 2014). When the blood has been collected in the syringe, the needle will be removed and the targeted area will be covered with cotton in purpose to stop the bleeding (Hicks R, 2013). Question 5 Provide one provisional diagnosis for the case study and one differential diagnosis (possible alternative). A provisional diagnosis is basically the first diagnosis or the working diagnosis that is made by a medical professional and it is usually not clarify as the final diagnosis[4]. This type of diagnosis is generally assign when the presenting problems meet some of the criteria for a disorder, but more information required for the accurate diagnosis and it might be modified as the patients care continues and more details of diagnosis presented[4]. Besides, the provisional diagnosis might change depending on the patients ongoing condition, his or her response to any treatments that is offered, and also the patients level of comfort with the new diagnosis. For this case the 40-year-old post-menopausal woman presents and claimed that she had a long term lethargy, loss of sex drive, abdominal pain for 2 months and discoloration of the skin. Based on the symptoms presented the most probable provisional diagnosis for her is Hemochromatosis as the symptoms presented are quite identical (Table 1). Hemochromatosis is the most common form of iron overload disease, in this cases the total iron body content recorded was 20g, where the normal iron levels for women ranges from 12 to 15.5g of hemoglobin per deciliter of blood[2]. Primary hemochromatosis or else known as hereditary hemochromatosis, is an inherited disease meanwhile, secondary hemochromatosis is caused by anemia, alcoholism, and other disorders[1]. The Hemochromatosis causes the body to absorb and store too much iron. Apart, the extra iron builds up in the bodys organs such as liver, heart and pancreas can lead to an organ damage[1]. Table 1 Patient Presented Symptoms and Hemochromatosis Symptoms Presented Symptoms Hemochromatosis Symptoms Long term lethargy Fatigue and lack of energy[1] Loss of sex drive Loss of sexual desire[1] Abdominal pain for 2 months Abdominal pain[1] Discoloration of skin Generalized darkening of skin color (Bronzing) [1] Loss of body hair[1] There is also another type of diagnosis and it is called a differential diagnosis or possible alternative. The differential diagnosis is the process of comparison among diseases exhibiting similar sign and symptoms[3]. When a patient has symptoms that are common to more than one disease or condition, the medical professional will make a list of the possibilities, and then eliminate them based on the symptoms that may not fit some of the possibilities[3]. In this case, when evaluating a patient with suspected hemochromatosis, alcoholic liver disease and multiple transfusions should also be considered[2]. Alcoholic liver disease Liver biopsy in the alcoholic disease patients may show a modest increase in iron. In contrary to patients with hemochromatosis, the hepatic iron levels alcoholic disease patients are relatively normal and iron stores are less than 4 g[2]. Multiple transfusions Hypertransfusion is performed in patients with sickle cell anemia, beta thalassemia major and also myelodysplastic syndrome[2]. Such patients may receive as many as 100 units of red blood cells, which may contain as much as 20-25 g of iron, similar to or more than the amount retained in many symptomatic patients with hereditary hemochromatosis[2]. References Mayoclinic.org. 2014. Hemochromatosis Symptoms Diseases and Conditions Mayo Clinic. [online] Available at: http://www.mayoclinic.org/diseases- conditions/hemochromatosis/basics/symptoms/CON-20023606 [Accessed: 26 Jan 2014]. Emedicine.medscape.com. 2014. Medscape: Medscape Access. [online] Available at: http://emedicine.medscape.com/article/177216-differential [Accessed: 26 Jan 2014]. Torrey, T. 2014. differential diagnosis. [online] Available at: http://patients.about.com/od/glossary/g/diffdiagnosis.htm [Accessed: 26 Jan 2014]. Schimelpfening, N. 2014. FAQ: What Is a Provisional Diagnosis Vs. Differential Diagnosis?. [online] Available at: http://depression.about.com/od/diagnosis/f/provisional.htm [Accessed: 26 Jan 2014]. Edward C. (2013). Histotechniques. Web Path. Retrieved January 26, 2014, fromhttp://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html Rolls Geoffey (2011). An Introduction to Specimen Preparation. Leica Biosystem. Retrieved January 26, 2014, from http://www.leicabiosystems.com/pathologyleaders/an- introduction-to-specimen-preparation/ Hubscher, S. (2008). Tissue Pathways for Liver Biopsies for the Investigation of Medical Disease and for Focal Lesions. E-med.co.il. 2014. [online] Available at: http://www.e-med.co.il/emed/new/Usersite/Presentations/Liver_Diseases/10.files/slide0014_image0 70.jpg [Accessed: 26 Jan 2014]. Library.med.utah.edu. 2014.Hepatic Pathology. [online] Available at: http://library.med.utah.edu/WebPath/LIVEHTML/LIVER018.html [Accessed: 26 Jan 2014]. Ukessays.co.uk. 2014.He stain and perls prussian blue technique. [online] Available at: http://www.ukessays.co.uk/essays/sciences/h-and-e-stain-and-perls-prussian-blue- technique.php [Accessed: 26 Jan 2014]. Rashmil, S. (n.d.). Retrieved from http://www.dako.com/index/knowledgecenter/kc_publications/kc_publications_conne ction/kc_publications_connection14.htm/28829_2010_conn14_special_stains_interpret ation_liver_biopsies_saxena.pdf Adams P (2008) Management Of Elevated Serum Ferritin Levels Journal. US National Library of Medicine, National Institute of Health (2008 May; 4(5):333-334. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093720, on January 27th 2014. Hicks R (2013) Ferritin blood test. Web MD. Retrieved from http://www.webmd.boots.com/a-to-z-guides/ferritin-blood-test, on January 26th 2014. KidsHealth (2014) Blood Test: Ferritin (Iron). Retrieved from http://kidshealth.org/parent/system/medical/test_ferritin.html#, on January 25th 2014. References: [4] Liver Pathology, In-text: (Google Books, 2014), Bibliography: Google Books. 2014. Liver Pathology. [online] Available at: http://books.google.com.my/books?id=worDZk6fSWwCpg=PA289lpg=PA289dq=why+used+perls+prussian+blue+to+detect+ironsource=blots=4RfzVoc3O5sig=BBCSmpM1Cwt6xMxrNBwH8c5hvU4hl=ensa=Xei=AkDmUpLRC8SprAfC_4DYBwredir_esc=y#v=onepageq=why used perls prussian blue to detect ironf=false [Accessed: 28 Jan 2014]. [5] Perls Prossian Blue Staining Protocol, In-text: (Ihcworld.com, 2014), Bibliography: Ihcworld.com. 2014. Perls Prossian Blue Staining Protocol. [online] Available at: http://www.ihcworld.com/_protocols/special_stains/perls_prussian_blue_ellis.htm [Accessed: 28 Jan 2014]. [6] Theory and Practice of Histological Techniques, In-text: (Google Books, 2014) Bibliography: Google Books. 2014. Theory and Practice of Histological Techniques. [online] Available at: http://books.google.com.my/books?id=Dhn2KispfdQCpg=PA235lpg=PA235dq=why+Perls+prussian+blue+is+stain+with+1%+neutral+redsource=blots=JyrzjwVyH8sig=9Qlm0_6T31awaDEuhGSChtQz3mQhl=ensa=Xei=04LmUsb8POW7iAf9uYGgBwredir_esc=y#v=onepageq=why Perls prussian blue is stain with 1% neutral redf=false [Accessed: 28 Jan 2014]. [7] He stain and perls prussian blue technique, In-text: (Ukessays.co.uk, 2014) Bibliography: Ukessays.co.uk. 2014. He stain and perls prussian blue technique. [online] Available at: http://www.ukessays.co.uk/essays/sciences/h-and-e-stain-and-perls-prussian-blue-technique.php#ixzz2rcLX4KTJ [Accessed: 28 Jan 2014]. [8] Perls’ Technique For The Demonstration of Haemosiderin – Method and Tips, In-text: (skinpathonline, 2011), Bibliography: skinpathonline. 2011. Perls’ Technique For The Demonstration of Haemosiderin – Method and Tips. [online] Available at: http://skinpathonline.wordpress.com/2011/06/20/perls’-technique-for-the-demonstration-of-haemosiderin-–-method-and-tips/ [Accessed: 28 Jan 2014]. [9] Perls M. Nachweis von Eisenoxyd in gewissen Pigmenten. Virchov’ s Arch Pat Anat und Phsiol und Klin Med 1867; 39: 42-48. Van Gieson I. Laboratory notes of technical methods for the nervous sistem. New York Med J 1889; 50: 57-60. A.F.I.P.. Laboratory Methods in Histotechnology. Washington D.C. A.F.I.P. 1994. [ Accessed : 28 Jan 2014]

sonnet 12 :: essays research papers

1. When I do count the clock that tells the time, 2. And see the brave day sunk in hideous night; 3. When I behold the violet past prime, 4. And sable curls, all silvered o'er with white; 5. When lofty trees I see barren of leaves, 6. Which erst from heat did canopy the herd, 7. And summer's green all girded up in sheaves, 8. Borne on the bier with white and bristly beard, 9. Then of thy beauty do I question make, 10. That thou among the wastes of time must go, 11. Since sweets and beauties do themselves forsake 12. And die as fast as they see others grow; 13. And nothing 'gainst Time's scythe can make defence 14. Save breed, to brave him when he takes thee hence. This sonnet is so famous that it almost makes commentary unessential. It will always be one of the best sonnets in the history of language. The lively and rapid passage of time, which brings every thing to an end, is described, not indeed in abundance, but with such noteworthy and overwhelming effect that humanity almost stares us in the face as we read it. The logic of the lines ends with the line itself is like the ticking of a clock or the unstoppable motion of a pendulum as it swings from side to side. The importance of the placing of this sonnet here (12) (I believe it’s because of the twelve hours of the day) as well as that of the 'minute' sonnet at (60) is hard to establish, but at the very least it points to an organized hand, which, like the clock itself, measures out the chain of important events as they occur. It is true, however, that it is not clear that we have Shakespeare’s order, so this is just my opinion. As for the forms of the sonnet, we are clear that it was definitely written by Shakespeare. A sonnet is a one-stanza poem of fourteen lines, written in iambic pentameter. One means to illustrate a verse line is to speak about how many stressed and unstressed syllables are in the line. A simple grouping of syllables, some stressed, some unstressed, is called a foot. The iambic foot is an unstressed syllable followed by a stressed syllable. Pentameter means there are five feet in the line. "Iambic Pentameter," subsequently, means a line of ten syllables, which interchanges unstressed and stressed syllables according to the iambic measure.

Working-Class Writer Essay -- Authors

David Herbert, D.H., Lawrence published his third novel, Sons and Lovers, in 1913. The story follows the Morel family. They live in â€Å"The Bottoms†, a small mining community in England, built over the remnants of â€Å"Hell Row†. The Morel family is unhappy, because of the relationship between Walter, the father, and Gertrude, the mother. Walter drinks too much, does little to provide Gertrude with emotional satisfaction, while Gertrude feels superior, and is unhappy with the family’s social status. Searching for emotional satisfaction, Gertrude turns to her children for comfort. At first, her affection focusses on the eldest son, William, but after he become ill and dies, Gertrude shifts her attention to Paul, the youngest son. There are many parallels between the novel and Lawrence’s own personal life. Similar to the Morel’s, the Lawrence’s family lived in a working-class community in Nottinghamshire, England. Lawrence’s father, Arthur, worked in a mine, and was a drinker. Lawrence’s older brother, Ernest, passed away early in life, causing Lawrence to become the focus of Lydia’s affection. Similar with Paul and Miriam Leivers, Lawrence had an early relationship with Jessie Chambers, of whom his mother did not approve. Like Paul’s relationship with Clara Dawes, Lawrence engaged in an affair with Alice Dax, a married woman with a family. Sons and Lovers is a semi-autobiographical novel in which Lawrence draws from his own personal relationships with his parents, his childhood sweetheart, and adult lover as the basis for his fictional characters, which he ultimately uses to build the foundation for his philosophical ideas regarding industrialism and love. In the story, Walter represents the embodiment of Lawrence’s issues ... ...d Walter Morel after his father, Arthur, and Gertrude Morel on his mother, Lydia. Furthermore, Lawrence represents himself in the protagonist, Paul Morel, the son of Walter and Gertrude Morel. Works Cited Becket, Fiona. D.H. Lawrence. London and New York: Routledge, 2002. 7-8. Print. Burgess, Anthony. Flame Into Being The Life and Work of D.H. Lawrence. New York: Arbor House, 1985. 57. Print. Bergquist, Carolyn. "Lawrence's Sons And Lovers." Explicator 53.3 (1995): 167-170. Academic Search Premier. Web. 9 May 2012. Kearney, Martin. Major Short Stories of D.H. Lawrence A Handbook. New York and London: Garland Publishing, Inc., 1998. 97. Print. Maddox, Brenda. D.H. Lawrence The Story of a Marriage. New York: Simon & Schuster, 1994. 63, 78, 93. Print. Worthen, John. D.H. Lawrence The Life of an Outsider. New York: Counterpoint, 2005. 30, 83. Print.

Poem Analysis “Out, Out”

Poem Analysis: â€Å"Out, Out-† In the poem, â€Å"Out, Out-â€Å", author Robert Frost starts off his poem by giving an inanimate object, the buzzsaw, a sense of life. Using the literary device, Personification, the buzz saw is being written with characteristics a curious and rather playful child. The buzzsaw acts like once hears the young man’s mother call for supper time, that it wants to eat, so eats the young man’s hand. The buzzsaw takes (Cuts Off) the hand in a rather subtle demeanor, but in truth, it would be a very graphic to behold.Throughout the poem, everything is written in a peaceful and quite tone, even during the violent and gruesome ones to. To add to the fact of the buzzsaw is being personified in the story, the buzzsaw seems to only attack when the mother calls all for supper. The buzzsaw acts like it knows what the meaning of supper time is. Another literary device used in this poem is the process of along with the use of otomotapia(s).Using repetition first to deliver emphasis to the reader of the sounds that buzzsaw would be making, and then the actual sound being written/sounded out in an otomotapia base. The otomotapia in the story would be the grinding sounds made the buzzsaw ripping the through the poor young man’s arm. This quote from the story pretty much sums all that I describe above; â€Å"The saw snarled and rattled, snarled and rattled†¦Ã¢â‚¬  and it continues about three more times over and over.The use of the sound effects gives the once playful buzzsaw a more animalistic approach, making it seem like it is hungry after hearing the key word â€Å"supper†. To conclude, the literary devices used in Robert Frost’s poem are mostly to emphasis and give life to once lifeless piece of machinery. The story, rather bloody and saddening, is a well written example of poetry and depth behind each and every letter/word. The analysis is still to be assessed, but this all gives basic underst anding as to what meant behind his more obvious literary devices.

Soccer Ritual

I had the passion to play soccer because of them and I was wanted to become as a famous player in Saudi Arabia Maxed Abdullah. From that moment my sibling and play it and like it very much. Also, we were fans to AY-Edited FCC as my father does because most of children want to become like their fathers. My father once bought for us a football, so my brother and I keep play with it and watch soccer matches to learn some skills. Furthermore, when we became good player, we start to play against my cousins and our neighbors.We won In all of games we took medallions. After that I was plan to win on my brothers and get the medallion from them to look a hero on my father eyes, so I started watch a lot soccer matches and soccer animation. I learn a lot of skills such as how to shoot by left foot, how to skip from the player, and how to pass the ball under the player and that happen when I was in second grade in elementary school. I start to collect my team from my cousins and my neighbors, so my oldest brother and I made a competition and we asked my father to Judge.So I won the game and my dad was surprised of the way I was played, so he gave me a hundred SIR is about $26 but in that time it value a lot because when my father gave us 5 RSI we feel like rich. Soccer is more than a sport; soccer player are a folk group with rituals and Informal tradition. In this research I will define a ritual and how is related to soccer. What Is a ritual? A ritual Is a particular type of tradition that many folklorist as a distinct category of folklore.Ritual are habitual actions, but they are more purposeful than customs rituals are frequently highly organized and controlled often ant to indicate or announce membership in a group. Most rituals are stylized, and highly conceptualized deeply symbolic activities that enable groups to acknowledge exemplify, and / or act out certain traditional ideas, values, and beliefs. Family and community celebrations scared and secular ceremonies, an d a variety of other structured performance include rituals.Rituals required a set of beliefs and values that group members accept and want to have reinforced. The ritual works to teach their importance by emphasizing even acting out these sales or belies like tradition in general. Rituals frequently employ symbols and metaphors to represent Important concepts. There are no clear records about who or whom Invited the soccer or the mime was, however the historian agreed that a kind of play ball was played for at least 3000 years, and that can see In China, Egypt, and Greece before modern one has developed in England.There are many rituals related to teams before they join first time. That was because they have a ritual before the game and they belief they can win the game. Especially Fabian Breather's shaved head before each game and hanging a towel on goal. Shatter is a soccer team in Khaki. Before they play the game they slaughtered a sheep to win games, however the animal rights g roup PETA .NET â€Å"strongly worded letter† to the FAA president. They do that because they feel the luck comes by this ritual.In many Muslim majority countries like Astrakhan, the ritual slaughter of a sheep is seen as bringing good fortune ahead of a major event. The meat is then often distributed among the poor. According to Shatter coach Smoky says, â€Å"Of course this tradition may have certain psychological impact on players that can help them to relax before the game. â€Å"(FOOTBALL) Slaughter a lamb has known from old centuries in many books and religions such as Islam, and Christianity. In holy Curran mention (e-*HCI 4439) that mean God command Abraham to sacrifice a sheep instead of his son Small (AY shaft 107).People sacrifice a sheep because they want to be placed by God or to forgive a sign, and some do it for the evil to make a bad thing. As Jewish and Muslim did the same thing when they have children. To be grateful to the God. Most soccer players have sup erstitions and rituals. They think they can success in their game. Superstitions can have variety things such as dirty socks, pre-game song, or a sort of pray. Also, there are funny operations like Christian Rolando has 7 1. On the bus Christian is sitting alone in the back row and he is the last one who leaves the bus 2. N the plane he sits always with Epee, and he is the first who leaves the plane 3. In the locker room at the halftime he must change his hairstyle 4. He must touch the ball before leaving the dressing room 5. In the Portugal national team he is the only Portuguese who is allowed to start off with a long-sleeve Jersey 6. On the pitch he always steps onto the right foot 7. When he scores the goal, first he is waving to his family (footballers). Sorcery Sorcery is a one of a taboo thing. Many clubs used sorcery to win the game.They used it before the game and some wear it or hang it. They do that that to blind the other team from scoring goals or they used devils to st op goalkeepers from follow the ball. According to AY hill FCC Ex player conference that he used sorcery in one of games by washing his face, and after the game he quit and went to Mecca to erase his sin. (sport kabob) In conclusion, soccer became a famous game in the world have it's on rituals and superstitions between the players, mangers, and fans. I

Past and Future

The article â€Å"Missing the past, embracing the future† by Gayatree Siddhanta Sarma is about a women who has a long-last childhood. However, her beautiful memories were brought back through the vacation with her daughter at the county fair. In addition, reading this article reminds us how important our childhood is that can directly influences our thoughts and behaviors. Therefore, our childhood memories play an importatn role in our adult lives. Even though few of us have the same childhood as the author’s, we have our own one which is either idyllic or complicated. According to the article, the author had a very idyllic and precious childhood which was full of happy memories with her dad. They planted trees, took care of their garden such as growing flawless cauliflower, 12-inch dahlias and organic food. Moreover, she also had her own corner plot to grow kohlrabi and sweet peas with a passion like her father. Besides, the spring came turning the garden into a fragant bouquet of flowers. All of the details in the article give us an idea that the author’s childhood was full of fresh and fragant air. Likewise, my childhood was lots of fun. In Vietnam, our neighborhood is close and friendly. In addition, our houses were nearby, so I could come to visit theirs even everyday. When I was young, my neighbors and I usually went around the area playing traditional games such as jumping-rope, hide and seek, and chopstick-ball which you have to catch the chopstick while the ball is in the air. Furthermore, I missed our New Year which is in mid-Febrary. We always gathered in my grandparent’s house to celebrate, get lucky money from our aunts and uncles, and play cards. Overall, those sweet memories affected our way of living now. While the author grows flowers with her daughter to recall her childhood, I teach my nices to play those games. One way or the other, we all remember our childhood memories by showing them to others in different methods. Other than that, I do believe our behaviors are affected by the way we were treated, and educated. As an English literature professor, the author’s father not only had passion for teaching but also for growing trees and flowers. Gently, he showed her how to grow a garden with full of beautiful flowers. In other words, one can not do that without patient, so her behaviors were built up since she was young. As a result, the author now know how to build her family traditionally although it is in a diffrent country. Similarly, I was grow up in a poor country, so all the games we played were communion. We did not have our own toys, but rope, balls, and chopticks which was from each of us. We shared and played together. Therefore, I leanred not to be selfish and jealous, but love others. In addition, I had many activities in church to help the elders, the orphange and the poor. All of that, I were leanred from my childhood memories. Our childhood memories totally influenced our lives today. It was a base for us to build a new future. Overall, it inspired us to create a beautiful childhood not only for our beloved ones but also for other kids.

Deliver, monitor and evaluate customer service to internal customers Essay

LEVEL 3 DIPLOMA IN BUSINESS & ADMINISTRATION Unit 328 – Deliver, monitor and evaluate customer service to internal customers – Knowledge Questions 1. Understand the meaning of internal customer 1.1Describe what is meant by internal customer Internal customers are people within your own organisation such as employers or colleagues who you provide a service for, e.g. 2. Know the types of products and services relevant to internal customers 2.1 Describe the products and services offered by own organisation to internal customers 3. Understand how to deliver customer service that meets or exceeds internal customer expectations 3.1 Explain the purpose and value of identifying internal customer needs and expectations 3.2 Explain why customer service must meet or exceed internal customer expectations 3.3 Explain the value of meeting or exceeding internal customers expectations 3.4 Explain the purpose and value of building positive working relationships 4. Understand the purpose of quality standards and timescales for delivering customer service 4.1 Identify quality standards for own organisation and work 4.2 Explain the value of agreeing quality standards and timescales 4.3 Explain how to set and meet quality standards and timescales with internal customers 5. Understand how to deal with internal customer service problems 5.1 Describe the types of problems that internal customers may have 5.2 Explain ways of dealing with problems 5.3 Explain the purpose and value of a complaints procedure, if applicable 6. Understand how to monitor and evaluate internal customer service and the benefits of this 6.1 Explain the purpose and benefits of monitoring internal customer satisfaction and how to do so 6.2 Describe techniques for collecting and evaluating customer feedback 6.3 explain the benefits of continuous improvement